(1)Every zoo shall endeavour to establish and sustain population of physically, genetically and behaviorally healthy animals for furthering the cause of wildlife conservation and communicating credible conservation message to the visitors through display of healthy animals in naturalistic settings.
(2)Every zoo shall, through proper landscaping and planting of appropriate species, provide a naturalistic environment within the zoo to enable the visitors to have communion with nature and get motivated for living in harmony with nature.
(3)Every zoo shall endeavour to regulate the movement of visitors in.the zoo in such a manner that zoo animals are not unduly disturbed, stressed or provoked and the zoo shall be closed to visitors at least one day ima week.
(4)Every zoo, as a safeguard against the un-regulated access of visitors to the zoo and animals being subjected to injury, pilferage and predation, shall design appropriately the barriers along the boundary of the zoo in accordance with the standards issued by the Central Zoo Authority in this regard.
(5)Every zoo, which is surrounded by human landscape shall be encompassed by a perimeter wall of at least two meter in height from the ground level on both sides.
(6)No residential colony shall be constructed within the zoo premises.Provided that where such colonies already exist, it shall be separated from the zoo premises by a boundary wall with a minimum height of two meters from the ground level. The entry to the residential colony shall not be through the zoo premises.
(7)Every zoo shall carry out its operations in a manner that causes minimum stress on natural resources and produces the minimum of solid wastes and effluents and the zoo shall also endeavour to put in place the practices of effective waste management through reducing, reusing and recycling and disposing the effluents and the solid wastes in a manner that causes least adverse environmental impact.
(8)Every zoo shall refrain from display of sick, injured, infirm and tethered animals to the visitors and such animals shall be housed in off-display facilities specially earmarked for and providing appropriate upkeep and healthcare for such animals.
(9)Every zoo shall refrain from housing of domestic animals and pets within the zoo premises and adequate safeguards shall also be put in place to prevent the entry of domestic livestock, stray animals and pets into the premises of the zoo.