(2)To safeguard against ill effects of inbreeding on the zoo population, the zoo shall endeavour to introduce unrelated animals to zoo stock through exchange, loaning and getting gifts of animals from other zoos.

(3)No zoo shall acquire single animal or genetically non-viable number of animals unless such acquisition is necessary for pairing of single animals or making the group genetically /biologically viable.

(4)Every zoo shall endeavour to acquire mates for single and unpaired animals on priority basis and in the event of any zoo failing to find a mate for single and unpaired animal within a period of six months, the unpaired or single animal shall be transferred or exchanged or given on breeding loan to any other zoo in accordance with the norms specified by the Central Zoo Authority in this regard.

(5)For the purpose of transportation of animals from one place to another, the standards specified in this regard by the Central Zoo Authority shall be complied with.

(6)The Central Zoo Authority shall assign the responsibility of conservation breeding of the identified critically endangered species to identified zoos having technical capabilities and housing facilities, preferably close to the distribution range of the species and every zoo shall help the identified zoos in implementing the breeding programme.

(7)The surplus animals from the breeding population shall be made available to the contributing zoo for display purposes on the basis of quality of housing and infrastructural facilities available with the zoos.

(8)Every zoo shall, with a view to prevent inbreeding and the loss of heterozygosity, the zoos shall maintain the animal history cards & stud and herd book for the animals of endangered species housed in the zoo and put appropriate identification marks and transfer or exchange animals with other zoos.

(9)Every zoo shall endeavour to limit the number of animals of each species within the limits set by the animal collection plan of the zoo by implementing appropriate population control measures like segregation of sexes, vasectomy, tubectomy and implantation of pellets, etc., with due consideration of the health and welfare of the animals and interest of their long term survival.

(10)Every zoo shall take effective measures to safeguard against escape of the zoo animals from the premises of the zoo and in the event of any accidental escape, immediate action shall be taken to retrieve the escaped animals.

(11)No zoo shall release any captive animal into the wild except in accordance with the norms specified by the Central Zoo Authority in this regard.

(12)Every zoo shall ensure that no hybridization of species or races of same species takes place in the zoo.